5 Tips about attune flow cytometer You Can Use Today
5 Tips about attune flow cytometer You Can Use Today
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Facts-enriched characterization of cell populations. Morphological facts from visuals can add for the richness of flow cytometry knowledge. By way of example, the figure exhibits an or else standard apoptosis assay using Annexin V and PI, adding cell imaging to characterize cells in Just about every populace to expose morphologically unique features. These insights couldn't are actually gained from multiplex staining alone.
As samples are obtained over the Attune CytPix Flow Cytometer, the superior-velocity brightfield digicam captures and outlets pictures of detected events at a amount as many as six,000 photos for every second, dependant upon the flow rate and impression sizing. For better adaptability, Attune Cytometric Software helps you to change the image seize frequency as necessary and select a particular gate for image capture, if needed.
(B) RAW 264.seven macrophages are frequently annotated inaccurately with respect to particle depend because of their complexity and noncircular borders. The quantity of singlets accessible for Assessment will increase once the user trains and employs a far more exact product.
The award-profitable Invitrogen Attune CytPix Flow Cytometer brings together acoustic concentrating having a superior-velocity brightfield digicam for simultaneous large throughput flow cytometry and superior resolution brightfield imaging.
"An image just isn't pretty worth 1000 dots, but it really helps to see pictures. It is great to find out photos that correlate with staining. One example is, AnnexinV-favourable cells which can be morphologically diverse than wholesome cells, or CD14+ monocytes that happen to be greater and much more textured than CD3+/CD4+/CD14- lymphocytes.
Here, an experienced consumer has gated singlets confidently. Right after assessing the guide singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate is made up of greater than four% aggregates.
Pair your Attune flow cytometer with an autosampler for amplified workflow performance, and include robotic integration for optimum throughput.
Our scientists will style and design panels to suit your needs as persistently you require—free of charge, no product or service purchase required.
Early indications of this contamination experienced to begin with been dismissed as particles. Backgating to find out new populations. Backgating imaged cells on the Attune CytPix Flow Cytometer also permits you to use morphological capabilities to find out attention-grabbing subpopulations that would not be apparent from flow cytometry data by itself.
Perhaps most of all, these activities incorporate cells of Obviously various phenotypes which may lead to incorrect conclusions relating to double optimistic events (particularly in scarce populations).
Sound waves help exact cell alignment, causing Extraordinary details top quality and ~10X more rapidly sample flow prices.
Application Notice: Automatic graphic analysis minimizes consumer-to-user variability in flow cytometry gating methods
Leverage the power of Innovative technological know-how and innovation to accelerate your discoveries. Have faith in within the Attune Flow Cytometer spouse and children to deliver the dependability, pace, and precision that established your work apart.
No wash/no lyse leukocyte Examination with imaging. Cells had been obtained from 24-hour-aged blood by dilution in one mM EDTA ( Specialist UX/UI enables customers to coach AI types for expanded applications. (A) The “Educate Design” attribute presents annotation equipment and authentic-time product schooling precision.
At the guts of each and every Attune Flow Cytometer lies our groundbreaking acoustic focusing technological know-how, which makes use of seem waves to meticulously align cells within the flow cell. This progressive technique surpasses common hydrodynamic strategies, resulting in a lot quicker processing speeds, Fantastic facts excellent, and increased sample throughput.
Since the mobile line had Beforehand been analyzed around the Attune CytPix Flow Cytometer, the scientists went back to the images and were being capable to document the microbial infection at least five days before.
The Attune CytPix Flow Cytometer lets people to visualize samples during acquisition, verify the precision of gating techniques, get more info and support benefits with responsible documentation for publication uses.
What appeared to be a homogeneous population is in fact a lot more varied—an Perception that ought to be deemed when interpreting effects.